HIF-1α (D2U3T) Rabbit mAb
货号：
14179S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 14179S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/ChIP |
| 使用方法 | WB(1:1000)
ChIP (1:50) |
性能| 供应商 | CST |
| 背景 | Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
参考图片Western blot analysis of extracts from various cell lines, untreated (-) or treated with either DMOG (1 mM, 6 hr; +) or cobalt chloride (100 nM, 4 hr; +) as indicated, using HIF-1α (D2U3T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
