| 货号 | 3414S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | HIF-1β/ARNT (C15A11) Rabbit mAb detects endogenous levels of total HIF-1β/ARNT protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 87 |
Confocal immunofluorescent analysis of MCF-7 cells using HIF-1β/ARNT (C15A11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).使用HIF-1β/ARNT (C15A11) 兔单抗（绿色）对MCF-7细胞进行激光共聚焦荧光分析。肌动蛋白丝使用Alexa Fluor® 554 鬼笔环肽（红色）标记。 | |
Western blot analysis of extracts from HepG2 cells using HIF-1β/ARNT (C15A11) Rabbit mAb.使用HIF-1β/ARNT (C15A11) 兔单抗对HepG2细胞提取物进行western blot分析。 |
