| 货号 | 12094S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | HMGA1 (D4F8) Rabbit mAb recognizes endogenous levels of total HMGA1 protein, isoforms 1a and 1b. Based on sequence homology, this antibody is not predicted to cross-react with HMGA2. |
| 使用方法 | WB(1:1000) IHC-P (1:2000) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | HMGA1, formerly known as HMG-I/Y, belongs to a family of high mobility group proteins that contain an AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA1 is highly expressed during embryogenesis and in embryonic stem cells, but not in fully differentiated adult tissues (2-4). Research studies have shown that HMGA1 is over-expressed in rapidly dividing neoplastic cells and a wide variety of aggressive cancers, including thyroid, colon, breast, pancreas, and prostate (2-4). Investigators have shown that forced expression of HMGA1 induces cellular transformation and an epithelial-to-mesenchymal transition (EMT), while inhibition of HMGA1 expression blocks anchorage-independent cell growth and proliferation of cancer cells, suggesting that HMGA1 contributes to carcinogenesis by inducing and maintaining a de-differentiated, highly proliferative cell state (5-8). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Confocal immunofluorescent analysis of NCCIT (high expression; left) and MCF7 (low expression; right) cells using HMGA1 (D4F8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).采用共聚焦免疫荧光术检测NCCIT（高表达；左）和MCF7细胞（低表达；右），使用的抗体为HMGA1 (D4F8) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。蓝色假彩色= DRAQ5® #4084 (荧光染料)。 | |
Western blot analysis of extracts from NCCIT and NTERA2 cl.D1 cells using HMGA1 (D4F8) Rabbit mAb.Western blot方法检测NCCIT 和 NTERA2 cl的提取物，使用的抗体为HMGA1 (D4F8) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HMGA1 (D4F8) Rabbit mAb.免疫组化检测石蜡包埋的人肺癌，所使用的抗体为HMGA1 (D4F8) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HMGA1 (D4F8) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).免疫组化分别检测在control peptide（左）或抗原特异性多肽（右）存在的情况下石蜡包埋的人结肠癌，所使用的抗体为HMGA1 (D4F8) Rabbit mAb。 |
