| 货号 | 8179S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | HMGA2 (D1A7) Rabbit mAb recognizes endogenous levels of total HMGA2 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (4-7). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (8-11). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Western blot analysis of extracts from various cell lines using HMGA2 (D1A7) Rabbit mAb. 使用HMGA2 (D1A7) Rabbit mAb兔单抗，免疫印迹(Western blot)分析不同细胞中HMGA2蛋白水平。 | |
Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with hTGF-β1 #8915 (10 ng/ml) and hIL-1β #8900 (5 ng/ml, 48 hr; right), using HMGA2 (D1A7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用HMGA2 (D1A7) Rabbit mAb兔单抗 (绿色)，共聚焦免疫荧光分析A549细胞，细胞分为untreated (左图)或处理hTGF-β1 #8915 (10 ng/ml)和hIL-1β #8900 (5 ng/ml, 48 hr; 右图)。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, HCT 116 (left) or HBP-ALL (right), using HMGA2 (D1A7) Rabbit mAb. 使用HMGA2 (D1A7) Rabbit mAb兔单抗，免疫组化分析HCT 116 (左图)或HBP-ALL (右图)石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HMGA2 (D1A7) Rabbit mAb. 使用HMGA2 (D1A7) Rabbit mAb兔单抗，免疫组化分析人源结肠癌石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human uterine leiomyoma using HMGA2 (D1A7) Rabbit mAb. 使用HMGA2 (D1A7) Rabbit mAb兔单抗，免疫组化分析人源子宫肌瘤石蜡切片。 |
