| 货号 | 6893S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | HMGB1 (D3E5) Rabbit mAb recognizes endogenous levels of total HMGB1 protein. This antibody does not cross-react with other HMGB proteins, including HMGB2 and HMGB3. |
| 使用方法 | WB(1:1000) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains (1,2). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-terminal acidic domain. HMGB1 is a widely expressed and highly abundant protein (2). HMGB2 is widely expressed during embryonic development, but is restricted to lymphoid organs and testis in adult animals (3). HMGB3 is only expressed during embryogenesis (4). While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of DNA, which results in local bending of the DNA double helix (1,2). HMGB proteins are recruited by and help facilitate the assembly of site-specific DNA binding proteins to their cognate binding sites in chromatin. For example, HMGB1 facilitates the binding of Hox proteins, Oct-1, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters (1,2). In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation (5,6). HMGB1 is massively released into the extracellular environment during cell necrosis, but not apoptosis. Extracellular HMGB1 "alarms" the innate immune system by acting as a chemoattractant for inflammatory leukocytes, smooth muscle cells, and stem cells, functioning as an immune adjuvant for soluble and particulate antigens, and triggering activation of T cells and dendritic cells. In addition, activated monocytes, macrophages and, dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. Hypoxia has also been shown to cause the release of HMGB1 in the liver, and some studies suggest a role for extracellular HMGB1 in tumor homeostasis (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 29 |
Western blot analysis of extracts from various cell lines using HMGB1 (D3E5) Rabbit mAb. 使用HMGB1 (D3E5) Rabbit mAb兔单抗，免疫印迹(Western blot)分析不同细胞中HMGB1 (D3E5)的蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcioma using HMGB1 (D3E5) Rabbit mAb. 使用HMGB1 (D3E5) Rabbit mAb兔单抗，免疫组化分析人源肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcioma using HMGB1 (D3E5) Rabbit mAb. 使用HMGB1 (D3E5) Rabbit mAb兔单抗，免疫组化分析人源结肠癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcioma using HMGB1 (D3E5) Rabbit mAb. 使用HMGB1 (D3E5) Rabbit mAb兔单抗，免疫组化分析人源前列腺癌组织石蜡切片。 |
