| 货号 | 12734S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | HMGN1 (D1I5O) Rabbit mAb recognizes endogenous levels of total HMGN1 protein. This antibody does not cross-react with other HMGN proteins. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:1000) |
| 供应商 | CST |
| 背景 | High mobility group (HMG) proteins are a superfamily of abundant and ubiquitous nuclear proteins that bind DNA without sequence specificity and induce structural changes to the chromatin fiber to regulate access to the underlying DNA. The HMGN family of proteins, which includes five members (HMGN1-5), is characterized by the presence of several conserved protein domains: a positively charged domain, a nucleosome binding domain, and an acidic C-terminal chromatin-unfolding domain (1,2). HMGN proteins function in transcriptional regulation and are recruited to gene promoters by transcription factors, such as estrogen receptor α (ERα), serum responsive factor (SRF), and PITX2, where they can facilitate either gene activation or repression (3-5). HMGN proteins bind specifically to nucleosomal DNA and reduce compaction of the chromatin fiber, in part by competing with linker histone H1 for nucleosome binding (6). In addition, HMGN proteins act to modulate local levels of post-translational histone modifications, decreasing phosphorylation of histone H3 at Ser10 and histone H2A at Ser1 and increasing acetylation of histone H3 at Lys14 (7-9). HMGN proteins can also modulate the activity of several chromatin-remodeling factors and restrict nucleosome mobility (10). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Confocal immunofluorescent analysis of NCCIT cells using HMGN1 (D1I5O) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).采用共聚焦免疫荧光术检测NCCIT细胞，使用的抗体为HMGN1 (D1I5O) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。 | |
Western blot analysis of extracts from various cell lines using HMGN1 (D1I5O) Rabbit mAb.Western blot方法检测不同细胞系的提取物，使用的抗体为HMGN1 (D1I5O) Rabbit mAb。 | |
Immunoprecipitation of HMGN1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HMGN1 (D1I5O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HMGN1 (D1I5O) Rabbit mAb.从 HeLa细胞提取物中免疫沉淀HMGA1，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane2)或HMGN1 (D1I5O) Rabbit mAb (lane 2)。Lane 1为10% input。 使用HMGN1 (D1I5O) Rabbit mAb进行Western blot检测。 |
