| 货号 | 3113S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | HNF4α (C11F12) Rabbit mAb detects endogenous levels of total HNF4α protein. |
| 使用方法 | WB(1:1000) IHC-P (1:200) IF-IC (1:6000) |
| 供应商 | CST |
| 背景 | Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
Confocal immunofluorescent analysis of HepG2 (left) and HeLa cells (right) using HNF4α (C11F12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).对HepG2细胞(左)和HeLa细胞(右)使用HNF4α (C11F12) Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor? 555 phalloidin（红色）标记。 | |
Immunohistochemical analysis of paraffin-embedded HepG2 cells in the presence of control peptide (left) or antigen specific peptide (right) using HNF4α (C11F12E8) Rabbit mAb.对石蜡包埋的HepG2细胞使用HNF4α (C11F12) Rabbit mAb抗体进行免疫组织化学分析，其中左图为对照肽，右图为抗原特异肽。 | |
Western blot analysis of extract from HepG2 cells using HNF4α (C11F12) Rabbit mAb.对HepG2细胞抽提液使用HNF4α (C11F12) Rabbit mAb进行Western blot分析。 | |
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma using HNF4α (C11F12) Rabbit mAb.对石蜡包埋的人肝细胞癌使用HNF4α(C11F12) Rabbit mAb进行免疫组化分析。 |
