| 货号 | 2623S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | HP1α/β (C7F11) Rabbit mAb detects endogenous levels of total HP1 α and HP1 β protein. The antibody does not cross-react with HP1 γ proteins. |
| 使用方法 | WB(1:1000) IP (1:25) IHC-P (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (α, β and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation (12-14). |
| 存放说明 | -20C |
| 计算分子量 | 25 |
Confocal immunofluorescent analysis of HeLa cells using HP1α (C7F11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用HP1α (C7F11) Rabbit mAb兔单抗 (绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HP1 α (C7F11) Rabbit mAb in the presence of control peptide (left) or HP1 alpha blocking peptide #1004 (right). 使用HP1 α (C7F11) Rabbit mAb兔单抗，其分别与control peptide (左图)或HP1 alpha blocking peptide #1004 (右图)孵育，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization using HP1 α (C7F11) Rabbit mAb. 使用HP1 α (C7F11) Rabbit mAb兔单抗，免疫组化分析人源肺癌组织石蜡切片，结果显示细胞核内定位。 | |
Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells using HP1 α (C7F11) Rabbit mAb. 使用HP1α (C7F11) Rabbit mAb兔单抗，免疫印迹(Western blot)分析HeLa, NIH/3T3, C6和COS细胞中HP1alpha蛋白水平。 |
