| 货号 | 15087S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) is a ubiquitously expressed, multidomain-containing protein that is tyrosine phosphorylated upon activation of multiple receptor tyrosine kinases (1). HRS contains a proline-rich region, which may mediate interactions with SH3 domain-containing proteins (1). Research studies have also demonstrated that HRS possesses a phosphatidylinositol 3-phosphate-binding FYVE-type zinc finger domain and a coiled-coil domain that target it to membranes of the endosomal compartment (2-4). HRS also possesses a ubiquitin-interacting motif (UIM) that binds ubiquitinated membrane proteins and, in conjunction with Eps15 and STAM proteins of the ESCRT-0 complex, facilitates their sorting through the endosomal compartment for eventual degradation in the lysosome (5-8). Research studies demonstrate that phosphorylation and ubiquitination of HRS play a role in EGFR intracellular trafficking and degradation (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Western blot analysis of extracts from various cell lines using HRS (D7T5N) Rabbit mAb. | |
Immunoprecipitation of HRS from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HRS (D7T5N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HRS (D7T5N) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left), treated with wortmannin #9951 (100 nM for 30 min, center) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml for 10 min, right) using HRS (D7T5N) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
