| 货号 | 4873S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rat IgG1 |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | HSP70 (6B3) Rat mAb detects endogenous levels of the inducible isoform of HSP70 protein. This antibody does not cross-react with other HSPs. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) F (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP and co-chaperone dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization using HSP70 (6B3) Rat mAb. 对石蜡包埋的人肺癌使用HSP70 (6B3) Rat mAb进行免疫组化分析，显示胞浆和胞核定位。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HSP70 (6B3) Rat mAb. 对石蜡包埋的人乳腺癌使用HSP70 (6B3) Rat mAb进行免疫组化分析。 | |
Western blot analysis of extracts from HeLa and COS cells, using HSP70 (6B3) Rat mAb. 对HeLa和COS细胞裂解液使用HSP70 (6B3) Rat mAb兔单抗进行Western blot分析。 | |
Flow cytometric analysis of Hela cells, using HSP70 (6B3) Rat mAb (blue) compared to a nonspecific negative control antibody (red). 使用 HSP70 (6B3) Rat mAb（蓝）和非特异的阴性对照抗体（红），对HeLa细胞进行流式细胞术分析。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat shock-treated (right), using HSP70 (6B3) Rat mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 对HeLa细胞，未处理（左）或热休克处理（右），使用HSP70 (6B3) Rat mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor®555 phalloidin（红色）标记。 |
