| 货号 | 4877S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | HSP90 (C45G5) Rabbit mAb detects endogenous levels of total HSP90 protein. This antibody does not cross-react with other HSPs. |
| 使用方法 | WB(1:1000) IHC-P (1:200) F (1:50) IF-IC (1:25) |
| 供应商 | CST |
| 背景 | HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP and co-chaperone dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Flow cytometric analysis of HeLa cells using HSP90 (C45G5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Immunohistochemical analysis of paraffin-embedded human colon using HSP90 (C45G5) Rabbit mAb. 对石蜡包埋的人结肠癌使用HSP90 (C45G5) Rabbit mAb兔单抗进行免疫组化分析。 | |
Confocal immunofluorescent analysis of HeLa cells using HSP90 (C45G5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 对HeLa细胞使用HSP90 (C45G5) Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。 |
