| 货号 | 9745S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | HtrA2/Omi (D20A5) Rabbit mAb recognizes endogenous levels of total HtrA2/Omi protein. This antibody does not cross-react with HtrA1. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | High temperature requirement protein A2 (HtrA2)/Omi is a serine protease with homology to the E. coli HtrA protein (DegP) and is thought to be involved in apoptosis and stress-induced degradation of misfolded proteins (1). While HtrA2 was orignally identified to be present in either the nucleus (1) or endoplasmic reticulum (2), subsequent studies have shown that it localizes in mitochondria and is released during apoptosis (3-8). HtrA2 is produced as a 50 kDa zymogen that is cleaved to generate a 36 kDa mature protein that exposes an amino terminal motif (AVPS) resembling that of the IAP inhibitor Smac/Diablo (3-8). Like Smac, interaction between HtrA2 and IAP family members, such as XIAP, antagonizes their inhibition of caspase activity and protection from apoptosis (3-8). Interestingly, HtrA2 knock-out mice did not show signs of reduced apoptosis, but rather had a loss of neurons in the striatum and a Parkinsons-like phenotype, suggesting that HtrA2 might have a neuroprotective function (9-11). This activity is associated with the protease activity of HtrA2 (9). Furthermore, research studies have shown that loss of function mutations in the HtrA2 gene are associated with Parkinsons disease (12). |
| 存放说明 | -20C |
| 计算分子量 | 36 |
Western blot analysis of extracts from various cell lines using HtrA2/Omi (D20A5) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为 HtrA2/Omi (D20A5) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a construct expressing full-length mouse HtrA2/Omi (mHtrA2/Omi; +), using HtrA2/Omi (D20A5) Rabbit mAb. Western blot方法检测293T细胞提取物，空质粒（-）或全长小鼠 HtrA2/Omi (mHtrA2/Omi; +)载体转染；使用抗体为HtrA2/Omi (D20A5) Rabbit mAb兔单抗。 | |
