| 货号 | 86630S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:400) F (1:800) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; right), using IDO (D5J4E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using IDO (D5J4E) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, overnight; right), using IDO (D5J4E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; (green) using IDO (D5J4E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using IDO (D5J4E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of IDO from extracts of HeLa cells treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IDO (D5J4E) Rabbit mAb. Western blot analysis was performed using IDO (D5J4E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IDO (D5J4E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using IDO (D5J4E) Rabbit mAb. |
