| 货号 | 3018S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | IGF-I Receptor beta (111A9) Rabbit mAb detects endogenous levels of total IGF-IR beta protein. It does not cross-react with insulin receptor. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Western blot analysis of extracts from various cell lines treated with insulin using IGF-I Receptor beta (111A9) Rabbit mAb. 对胰岛素处理的多个细胞系使用IGF-I Receptor beta (111A9) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunoprecipitation of IGF-I Receptor beta from untreated mIMCD3 cell extracts using IGF-I Receptor beta (111A9) Rabbit mAb (Lane 1). Lane 2: No antibody control. Lane 3: Input control. 对未处理的mIMCD3细胞裂解液使用IGF-I Receptor beta (111A9) Rabbit mAb兔单抗（列1）进行IGF-I Receptor beta免疫共沉淀分析。列2：无抗体对照。列3：输入对照。 |
