| 货号 | 14534S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 95, 200 |
Immunohistochemical analysis of paraffin-embedded MCF7 (left) and SK-UT-1 (right) cell pellets using IGF-I Receptor β (D4O6W) Rabbit mAb (IHC Preferred). | |
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using IGF-I Receptor β (D4O6W) Rabbit mAb (IHC Preferred). | |
Immunohistochemical analysis of paraffin-embedded human papillary serous cystadenocarcinoma of the ovary using IGF-I Receptor β (D4O6W) Rabbit mAb (IHC Preferred). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using IGF-I Receptor β (D4O6W) Rabbit mAb (IHC Preferred). | |
Western blot analysis of extracts from MCF7 (IGF-I receptor β positive), 293 (IGF-I receptor β positive), and SK-UT-1 (IGF-I receptor β negative) cells using IGF-I Receptor β (D4O6W) Rabbit mAb (IHC Preferred) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
