| 货号 | 8943S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | IKKβ (D30C6) Rabbit mAb recognizes endogenous levels of total IKKβ protein. This antibody does not cross-react with other IKK family members. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 计算分子量 | 87 |
Western blot analysis of extracts from various cell lines using IKKβ (D30C6) Rabbit mAb.Western免疫印迹。用IKKβ (D30C6) Rabbit mAb研究各种细胞系的细胞提取液。 | |
Western blot analysis of extracts from wild-type, IKKα (-/-), and IKKβ (-/-) mouse embryonic fibroblasts (MEFs) using IKKβ (D30C6) Rabbit mAb (upper) and GAPDH (14C10) Rabbit mAb #2118 (lower).Western免疫印迹。用 IKKβ (D30C6) Rabbit mAb (上图) 和 GAPDH (14C10) Rabbit mAb #2118 (下图)研究野生型的IKKα (-/-)和IKKβ (-/-)小鼠胚胎成纤维细胞(MEFs)的细胞提取液。 |
