| 货号 | 12242S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P |
| 目标/特异性 | IL-1β (3A6) Mouse mAb recognizes endogenous levels of total IL-1β protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. |
| 存放说明 | -20C |
| 计算分子量 | 17,31 |
Western blot analysis of 1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 and 1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204 using IL-1β (3A6) Mouse mAb.Western blot分析1 ng recombinant Human Interleukin-1β (hIL-1β) #8900 和1 ng recombinant Mouse Interleukin-1β (mIL-1β) #5204，使用抗体是IL-1β (3A6) Mouse mAb。 | |
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +) and LPS (100 ng/mL, 6 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析Raw264.7细胞的细胞提取物，未处理(-)或使用Brefeldin A #9972 (300 ng/mL, 刺激持续3小时; +) 和 LPS (100 ng/mL, 6 hr; +)处理，使用抗体是IL-1β (3A6) Mouse mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/mL, 3 hr; +), using IL-1β (3A6) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析THP-1细胞的细胞提取物，未处理(-)或LPS-处理 (100 ng/mL, 3 小时; +)，使用抗体是IL-1β (3A6) Mouse mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Immunohistochemical analysis of paraffin-embedded human large intestine (chronic colitis of the colon) using IL-1-β (3A6) Mouse mAb. | |
Immunohistochemical analysis of paraffin embedded THP-1 cell pellets, control (left) or LPS-treated (right) using IL-1-β (3A6) Mouse mAb. | |
Immunohistochemical analysis of paraffin-embedded human large intestine (ulcerative chronic colitis of the rectum) using IL-1-β (3A6) Mouse mAb. |
