| 货号 | 12239S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | IL-2 (D7A5) Rabbit mAb recognizes endogenous levels of total human IL-2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated (+) overnight with TPA #4174 (40 nM), A23187 (2 μM), and Brefeldin A #9972 (BFA, 300 ng/ml, 45 min delay), using IL-2 (D7A5) Rabbit mAb.Western blot分析JurKat细胞的细胞提取物，未处理(-)或TPA #4174 (40 nM）A23187 (2 μM), 和 Brefeldin A #9972 (BFA, 300 ng/ml, 45 min 延迟)处理过夜(+)，使用抗体是IL-2 (D7A5) Rabbit mAb。 | |
Western blot analysis of recombinant Human Interleukin-2 (hIL-2) (mammalian derived) #5454 using IL-2 (D7A5) Rabbit mAb.Western blot分析recombinant Human Interleukin-2 (hIL-2) (mammalian derived) #5454 ，使用抗体是IL-2 (D7A5) Rabbit mAb。 |
