| 货号 | 12153S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Acute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial, and other cells (1,2). IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes, and T cells. IL-6, in concert with TGF-β, is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/Stat cascade and the SHP-2/Erk MAP kinase cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is nonmembrane-associated (3).The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Rα (3). Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer (5). |
| 存放说明 | -20C |
| 计算分子量 | 21-28 |
Immunoprecipitation of IL-6 from NCI-H460 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D3K2N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D3K2N) Rabbit mAb.对NCI-H460细胞提取物中的IL-6进行免疫共沉淀，使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 IL-6 (D3K2N) Rabbit mAb (泳道3)。泳道1是10%的加入量。Western blot 分析使用抗体为IL-6 (D3K2N) Rabbit mAb。 | |
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (100 ng/ml, 6 hr; +) and treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +), using IL-6 (D3K2N) Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析通过TPA #4174 (80 nM,过夜)分化的THP-1细胞的细胞提取物，未处理(-)或LPS-处理(100 ng/ml, 6小时; +)以及Brefeldin A #9972 (300 ng/mL,持续刺激3h; +)处理；使用抗体是IL-6 (D3K2N) Rabbit mAb或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Western blot analysis of 1 ng recombinant Human Interleukin-6 (hIL-6) #8904 using IL-6 (D3K2N) Rabbit mAb.Western blot分析 1 ng recombinant Human Interleukin-6 (hIL-6) #8904 ，使用抗体是IL-6 (D3K2N) Rabbit mAb。 |
