| 货号 | 3856S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/ZebrafishBovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | ILK1 (4G9) Rabbit mAb detects endogenous levels of total ILK1 protein. The antibody may cross-react with ILK2. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of cell extracts from various cell lines, using ILK1 (4G9) Rabbit mAb.使用ILK1（4G9）兔单抗对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® ILK1 siRNA I #6202 or SignalSilence® ILK1 siRNA II (+), using ILK1 (4G9) Rabbit mAb #3856 and β-Actin Antibody #4967. ILK1 (4G9) Rabbit mAb confirms silencing of ILK1 expression and β-Actin Antibody is used to control for loading and specificity of ILK1 siRNA.转染100nM的SignalSilence®对照组siRNA（荧光标记）#6201（-）或SignalSilence® ILK1 siRNA I #6202或SignalSilence® ILK1 siRNA II (+)后的HeLa细胞提取物，使用ILK1 (4G9)兔单抗#3856和β-Actin 抗体#4967进行western blot分析。ILK1 (4G9)兔单抗用以确认ILK1的沉默表达，β-Actin抗体用作上样对照和ILK1 siRNA的特异性对照。 |
