| 货号 | 3020S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | Insulin Receptor beta (L55B10) Mouse mAb detects endogenous levels of total insulin receptor β. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Western blot analysis of cell lysates from CHO and CHO/IR transfected with insulin receptor expression cDNA, using Insulin Receptor β (L55B10) Mouse mAb. 对CHO和过表达胰岛素受体的CHO/IR细胞裂解液使用Insulin Receptor β (L55B10) Mouse mAb鼠单抗进行Western blot分析。 |
