| 货号 | 9876S |
| 反应种属 | Mouse |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC |
| 目标/特异性 | IRAP (3E1) Mouse mAb recognizes endogenous levels of total IRAP protein. |
| 使用方法 | WB(1:1000) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | IRAP (also known as LNPEP) was originally described as an insulin-responsive aminopeptidase found in Glut4-containing vesicles (1). It is essentially always in the same compartments as Glut4 and has identical insulin-stimulated translocation patterns as Glut4 (2). IRAP is therefore considered to be a surrogate marker for Glut4 (2). IRAP was later found to be a critical enzyme that regulates the expression and activity of several essential hormones and regulatory proteins, including the Glut4 transporter (3,4). This membrane associated, zinc-dependent cystinyl aminopeptidase acts as both a receptor for angiotensin IV as well as the enzyme that catalyzes the synthesis of this essential hormone from its angiotensinogen precursor (5). IRAP catalyzes the hydrolysis of several peptide hormones, including oxytocin and vasopressin (4). Abnormal IRAP expression or activity is associated with several forms of cancer in humans, including renal and endometrial cancers (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 165 |
Confocal immunofluorescent analysis of differentiated 3T3-L1 cells, treated with LY294002 #9901 (50 μM, 2hr; left) or insulin (100 nM, 30 min; right), using IRAP (3E1) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from 3T3-L1 cells using IRAP (3E1) Mouse mAb. |
