| 货号 | 4302S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | IRF-3 (D83B9) Rabbit mAb detects endogenous levels of total IRF-3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2). |
| 存放说明 | -20C |
| 计算分子量 | 45-55 |
Western blot analysis of extracts from COS-7 cells, mock transfected or transfected with human, mouse or rat IRF-3 constructs, using IRF-3 (D83B9) Rabbit mAb. The human and mouse constructs contain epitope tags.Western免疫印迹。用IRF-3 (D83B9) Rabbit mAb研究未转染和转染了人，小鼠或大鼠IRF-3质粒的 COS-7细胞的细胞提取液。人和小鼠的质粒包含了抗原决定部位标签。 | |
Western blot analysis of extracts from differentiated THP-1 cells, untreated or treated with LPS (1 mu-g/ml, 1 hour), using Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947 (upper) or IRF-3 (D83B9) Rabbit mAb (lower).Western免疫印迹。用Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947(上图)或IRF-3 (D83B9) Rabbit mAb(下图)研究未经处理的和经 LPS (1 μg/ml，1小时)处理分化了的THP-1细胞的细胞提取液。 | |
Western blot analysis of extracts from various cell lines using IRF-3 (D83B9) Rabbit mAb.Western免疫印迹。用IRF-3 (D83B9) Rabbit mAb研究各种细胞的细胞提取液。 |
