| 货号 | 4299S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | IRF-4 (D43H10) Rabbit mAb detects endogenous levels of total IRF-4 protein. Both alternatively spliced isoforms are detected. The antibody does not cross-react with other family members at physiological levels. |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:100) |
| 供应商 | CST |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).干扰素调控因子(IRFs) 包含一个家族的转录调控因子，这个家族主要是在 Jak/Stat信号通路中起作用，在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌，自免疫，淋巴细胞发育，细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都有同源性。IRF家族成员通过与具有相似的DNA-结合区域的蛋白的反应而调控转录，例如IFN刺激的响应原件(ISRE), IFN consensus序列 (ICS) 和IFN 调控元件(IRF-E) (2)。 |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of extracts from various cell lines using IRF-4 (D43H10) Rabbit mAb.Western免疫印迹。用IRF-4 (D43H10) Rabbit mAb研究各种细胞系的细胞提取液。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 H929 cells and either 5 μl of IRF-4 (D43H10) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human SUB1 Promoter Primers #5156 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
