| 货号 | 9247S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) detects endogenous levels of total IκBα protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | -20C |
| 计算分子量 | 39 |
Western blot analysis of extracts from HT29 cells (lane 1), protein A alone immunoprecipitation (lane 2), immunoprecipitation with nonspecific mouse IgG2a (lane 3), or immunoprecipitation with IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lane 4). Western blot was performed using IκBα Antibody #9242.Western免疫印迹，用IκBα Antibody #9242研究HT29细胞的细胞提取液 (泳道 1), 只用protein A的免疫沉淀反应(泳道 2), 用非特异性的鼠抗IgG2a免疫沉淀反应(泳道 3), 用IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen)抗体免疫沉淀反应(泳道 4)。 | |
Western blot analysis of extracts from Hela cells, treated with TNF-α (#2169, 10 ng/ml) for the indicated times, using Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (upper) or IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lower).Western免疫印迹。用Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (上图) 或者 IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen)(下图)研究经TNF-α (#2169, 10 ng/ml)处理一定时间的Hela细胞的细胞提取液。 |
