IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)【科瑞奥博】

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IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)

货号： 4814S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	4814S
反应种属	Human/Mouse/Rat/Monkey/Bovine/Pig/GuineaPig
来源宿主	Mouse
应用	W/IP/IHC-P/IF-IC/F
目标/特异性	IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) detects endogenous levels of total IκBα protein.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:100) F (1:800) IF-IC (1:400)

性能
供应商	CST
背景	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
存放说明	-20C
计算分子量	39

参考图片

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)。

Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).免疫组织化学染色分析石蜡包埋人肾腺癌组织。所用抗体为IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)。

Immunohistochemical analysis of paraffin-embedded human leiomyoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).免疫组织化学染色分析石蜡包埋人平滑肌瘤组织。所用抗体为IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)。

Flow cytometric analysis of HeLa cells, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (blue) compared to a nonspecifc negative control antibody (red).流式细胞仪研究HeLa细胞，所用抗体为IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (蓝色) 与非特异性阴性对照抗体 (红色)。

Western blot analysis of extracts from HeLa, NIH/3T3 and PC12 cells, using IkBα (L35A5) Mouse mAb (Amino-terminal Antigen).Western免疫印迹。用IkBα (L35A5) Mouse mAb (Amino-terminal Antigen)研究HeLa, NIH/3T3 和 PC12 细胞的细胞提取液。

Confocal immunofluorescent analysis of HeLa cells, untreated (left), or TNF-α-treated (#8902, 10 ng/ml for 15 min, right) using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析未经处理(左图)或经TNF-α-处理(#8902, 10 ng/ml ,15 min, 右图)的HeLa细胞，所用抗体为IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (红色)。Blue pseudocolor = DRAQ5® #4084 ( DNA 荧光染料)。

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246 (upper) and IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (lower).Western免疫印迹。用Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246 (上图) 和IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (下图)研究经TPA (#9905, 80 nM,24h)分化并经1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。