| 货号 | 2620S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Jagged1 (28H8) Rabbit mAb detects endogenous levels of total Jagged1 protein. It does not cross-react with Jagged2. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
Western blot analysis of total cell lysates from HepG2 and LNCaP cells, using Jagged1 (28H8) Rabbit mAb. 使用Jagged1 (28H8) 兔单抗对HepG2和LNCaP细胞全细胞提取物进行western blot分析。 |
