| 货号 | 3393S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | JMJD2A (C70G6) Rabbit mAb detects endogenous levels of total JMJD2A protein. This antibody does not cross-react with other Jumonji C proteins, including JMJD2B, JMJD2C and JMJD2D. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The JMJD2 (Jumonji domain-containing protein 2) family, also known as JHDM3 (JmjC domain-containing histone demethylation protein 3) family, contains four members: JMJD2A/JHDM3A, JMJD2B/JHDM3B, JMJD2C/JHDM3C and JMJD2D/JHDM3D. In addition to the JmJC domain, these proteins also contain JmJN, PHD and Tudor domains, the latter of which has been shown to bind to methylated histone H3 at Lys4 and Lys9, and methylated histone H4 at Lys20 (4,5). JMJD2 proteins have been shown to demethylate di- and tri-methyl histone H3 at Lys9 and Lys36, and function as both activators and repressors of transcription (6-11). JMJD2A, JMJD2C and JMJD2D function as coactivators of the androgen receptor in prostate tumor cells (7). In contrast, JMJD2A also associates with Rb and N-CoR corepressor complexes and is necessary for transcriptional repression of target genes (8,9). JMJD2B antagonizes histone H3 Lys9 tri-methylation at pericentric heterochromatin (10). JMJD2C, also known as GASC1, is amplified in squamous cell carcinomas and metastatic lung carcinoma, and inhibition of JMJD2C expression decreases cell proliferation (11,12). JMJD2C has also been identified as a downstream target of Oct-4 and is critical for the regulation of self-renewal in embryonic stem cells (13). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
Western blot analysis of extracts from NCCIT, NIH/3T3 and H-4-II-E cells using JMJD2A (C70G6) Rabbit mAb. 使用JMJD2A (C70G6) Rabbit mAb兔单抗，免疫印迹(Western blot)分析NCCIT、NIH/3T3和H-4-II-E细胞中JMJD2A (C70G6)的蛋白水平。 | |
Confocal immunofluorescent analysis of NCCIT cells using JMJD2A (C70G6) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用JMJD2A (C70G6) Rabbit mAb 兔单抗(绿色)标记，共聚焦免疫荧光分析NCCIT细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
