| 货号 | 3753S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:800) IF-IC (1:200) ChIP (1:50) |
| 供应商 | CST |
| 背景 | JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 42, 43 |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using JunB (C37F9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学方法检测石蜡包埋的人肺癌组织，使用的抗体为JunB (C37F9) Rabbit mAb。左侧为对照组多肽，右侧为抗原特异性多肽。 | |
Confocal immunofluorescent analysis of MCF-7 cells using JunB (C37F9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测： MCF-7细胞，标记的抗体是 JunB (C37F9) Rabbit mAb(绿色)。肌动蛋白丝用Alexa Fluor® 555 鬼笔环肽(红色)标记。 | |
Western blot analysis of extracts from various cell lines using JunB (C37F9) Rabbit mAb.Western blot检测细胞提取物：各种细胞系，使用的抗体是JunB (C37F9) Rabbit mAb。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of JunB (C37F9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
