| 货号 | 13243S |
| 反应种属 | Human/Monkey/Dog |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | <p>Kinectin 1 (KTN1) is an endoplasmic reticulum (ER)-enriched integral membrane protein that may be involved in the formation of ER sheets (reviewed in 1). Kinectin 1 binds the microtubule motor protein kinesin and acts as a membrane anchor for kinesin-based organelle trafficking (2). The interaction of kinesin with kinectin 1 has been shown to affect ER-supported focal adhesion assembly (3). Kinectin 1 has also been implicated in translation elongation, as an anchor for the elongation factor complex to the ER (4,5). Research investigators have shown that kinectin 1 expression is altered in multiple human pathologies, including breast cancer (6), hepatocellular carcinoma (HCC) (7), Parkinsons disease (8), and the autoimmune syndrome Behçets disease (BD) (9,10).</p> Kinectin 1 (KTN1)是一个内质网（ER）富集的整合膜蛋白可能涉及了ER薄片的形成(reviewed in 1). Kinectin 1和微管马达蛋白kinesin结合，作为一个基于kinesin的细胞器追踪的细胞膜锚定（2）。Kinesin和kinectin1的相互作用被认为会影响ER支持的黏着斑组装（3）。Kinectin1也被认为在翻译延长过程中发挥作用，为延长因子复合物向ER锚定（4,5）。研究人员证明kinectin1的表达在多种人类病理状态下都会发生改变，包括乳腺癌（6），肝细胞癌（HCC）（7），帕金森氏病（8），以及自身免疫综合征Behçets疾病（BD）（9,10）。 |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Confocal immunofluorescent analysis of SK-OV-3 cells using Kinectin 1 (D5F7J) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Kinectin 1 (D5F7J) Rabbit mAb (绿色)对SK-OV-3细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DyLight™ 554 Phalloidin #13054 (红色)标记。蓝色假色= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from T24, HeLa, and COS-7 cells using Kinectin 1 (D5F7J) Rabbit mAb.使用Kinectin 1 (D5F7J) Rabbit mAb对T24，HeLa和COS-7细胞提取物进行western blot分析。 | |
Immunoprecipitation of kinectin 1 from T24 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Kinectin 1 (D5F7J) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Kinectin 1 (D5F7J) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 Kinectin 1 (D5F7J) Rabbit mAb (lane 3)对T24细胞提取物中的kinectin 1进行免疫沉淀实验。Lane1是10%上样量。 使用Kinectin 1 (D5F7J) Rabbit mAb进行western blot分析。 |
