| 货号 | 13776S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The KiSS-1 receptor (KISS1R, GPR54) is a G protein-coupled receptor that inhibits cancer cell metastasis and plays a major role in gonadotropic axis physiology (1). The GPR54 protein was originally described as an orphan receptor homologous to the galanin receptor, and later identified as a receptor for amidated peptide products of the metastasis suppressor gene KiSS-1 (2,3). In humans, amidated kisspeptin ligands are produced predominantly in cells of the arcuate nucleus and preoptic area, with expression controlled by gonadal hormones (4). Research studies show that deletion of either the KiSS-1 receptor or KiSS-1 genes leads to hypogonadotropic hypogonadism, a disorder characterized by reduced levels of circulating testosterone and gonadotropins, as well as abnormal sexual maturation (5,6) The administration of kisspeptins potently stimulates gonadotropin secretion, indicating that KISS1R and kisspeptins play a major role in the physiology of the gonadotropic axis (7). Additional research demonstrates that KISS1R and kisspeptins inhibit metastasis in cancer cells by inhibiting cell motility (8). However, other studies indicate that increased expression of KISS1R and its ligands in human breast tumors correlates with higher tumor grade and metastatic potential, likely by engaging MMP-9 activation via transactivation of EGFR (9). |
| 存放说明 | -20C |
| 计算分子量 | 40-140 |
Western blot analysis of extracts from various tissues and cell lines using KISS1R (D9D7C) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of KISS1R from ZR-75-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or KISS1R (D9D7C) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using KISS1R (D9D7C) Rabbit mAb. |
