| 货号 | 2180S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) IF-IC (1:400) IHC-F (1:800) |
| 供应商 | CST |
| 背景 | Ku is a heterodimeric protein composed of two subunits (Ku70 and Ku80) originally identified as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis and systemic lupus erythematosus (1). Ku is an abundant, ubiquitously expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks (2-5). The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). DNA-PK has been shown to phosphorylate many proteins, including p53, serum response factor, c-Jun, c-Fos, c-Myc, Oct-1, Sp-1 and RNA polymerase II (1,8). The combined activities of Ku70/Ku80 and DNA-PK implicate Ku in many cellular functions, including cell cycle regulation, DNA replication and repair, telomere maintenance, recombination and transcriptional activation.Ku是由两个亚基(Ku70 and Ku80)组成的异源二聚体蛋白，最初在几种自体免疫疾病包括硬皮病、多发性肌炎和系统性红斑狼疮中作为自体抗原被鉴定出来(1)。Ku是一种高丰度、广泛表达的核蛋白，它能够结合并稳定端粒或双链DNA断裂处的DNA末端(2-5)。Ku70/Ku80异源二聚体具有ATP-依赖的DNA解旋酶活性，并能够作为DNA-依赖蛋白激酶(DNA-PK)的DNA-结合调节成分发挥作用(6-8)。DNA末端DNA-PK复合体的装配需要非同源末端连接(NHEJ)，一种涉及双链DNA断裂修复和V(D)J重组的机制(8)。DNA-PK能够磷酸化许多蛋白，包括p53，血清反应因子，c-Jun，c-Fos，c-Myc，Oct-1，Sp-1 和RNA 聚合酶 II (1,8)。Ku70/Ku80和DNA-PK的联合活性暗示了Ku在多种细胞功能中的作用，包括细胞周期调控、DNA复制和修复、端粒维持、重组和转录激活。 |
| 存放说明 | -20C |
| 计算分子量 | 86 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Ku80 (C48E7) Rabbit mAb.免疫组织化学方法检测石蜡包埋人类结肠癌组织，使用的抗体为Ku80 (C48E7) Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells using Ku80 (C48E7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦免疫荧光方法检测HeLa细胞，使用的抗体为 Ku80 (C48E7) Rabbit mAb，呈绿色。肌动蛋白纤维用 Alexa Fluor® 555 鬼笔环肽标记，呈红色。 | |
Western blot analysis of cell extracts from HeLa, A549 and COS cells using Ku80 (C48E7) Rabbit mAb.Western blot 方法检测HeLa, A549 和COS细胞提取物，使用的抗体为 Ku80 (C48E7) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human glioblastoma using Ku80 (C48E7) Rabbit mAb.免疫组织化学方法检测石蜡包埋人类胶质母细胞瘤切片，使用的抗体为 Ku80 (C48E7) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human GIST using Ku80 (C48E7) Rabbit mAb.免疫组织化学方法检测石蜡包埋人类胃肠间质瘤组织切片，使用的抗体为Ku80 (C48E7) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human melanoma using Ku80 (C48E7) Rabbit mAb.免疫组织化学方法检测石蜡包埋人类黑素瘤组织，使用的抗体为Ku80 (C48E7) Rabbit mAb。 | |
Immunohistochemical analysis of frozen SKOV-3 xenograft using Ku80 (C48E7) Rabbit mAb. |
