| 货号 | 4777S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-F/IF-IC/F |
| 目标/特异性 | Lamin A/C (4C11) Mouse mAb detects endogenous levels of lamin A and lamin C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2. |
| 使用方法 | WB(1:2000) IP (1:50) IHC-P (1:200) F (1:200) IF-F (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 74 (Lamin A), 63 (Lamin C) |
Western blot analysis of extracts from various cell lines using Lamin A/C (4C11) Mouse mAb. | |
Western免疫印迹分析各种细胞的细胞提取液，用的抗体是Lamin A/C (4C11) Mouse mAb 鼠单抗。 | |
Western blot analysis of extracts from THP-1 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by TNF-α #8902 (20 ng/ml, 4 hours), using Lamin A/C (4C11) Mouse mAb. | |
Western免疫印迹分析THP-1细胞的细胞提取液，未处理和cycloheximide (CHX, 10 μg/ml, 过夜)处理（随后用TNF-α #8902 (20 ng/ml, 4 小时))，使用 Lamin A/C (4C11) Mouse mAb 鼠单抗。 |
