| 货号 | 12255S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Lamin B2 (D8P3U) Rabbit mAb recognizes endogenous levels of total lamin B2 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, arising from alternative splicing. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene is correlated with the pathogenesis of the neurological disorder adult-onset leukodystrophy (10). |
| 存放说明 | -20C |
| 计算分子量 | 68 |
Confocal immunofluorescent analysis of HCT 116 cells using Lamin B2 (D8P3U) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HCT 116 细胞，使用抗体为 Lamin B2 (D8P3U) Rabbit mAb 兔单抗（绿色）。DY-554 phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 | |
Western blot analysis of extracts from various cell lines using Lamin B2 (D8P3U) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为 Lamin B2 (D8P3U) Rabbit mAb 兔单抗。 | |
Immunoprecipitation of lamin B2 from Jurkat cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Lamin B2 (D8P3U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Lamin B2 (D8P3U) Rabbit mAb. 免疫沉淀方法检测Jurkat 细胞提取物中 lamin B2 ，抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或Lamin B2 (D8P3U) Rabbit mAb 兔单抗(泳道3)。泳道1为10%对照。使用Lamin B2 (D8P3U) Rabbit mAb 兔单抗进行Western blot分析。 |
