LC3C (D3O6P) Rabbit mAb
货号：
14736S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 14736S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11). |
| 存放说明 | -20C |
| 计算分子量 | 14 |
参考图片Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing full-length human LC3A protein (hLC3A; +), human LC3B protein (hLC3B; +), or human LC3C protein (hLC3C; +), using LC3C (D3O6P) Rabbit mAb. |
Western blot analysis of extracts from A172 and HOP-92 cells, untreated (-) or chloroquine-treated (50 μM, overnight; +), using LC3C (D3O6P) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). |
