| 货号 | 3582S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | LDHA (C4B5) Rabbit mAb detects endogenous levels of total LDHA protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1-α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from various cell types using LDHA (C4B5) Rabbit mAb. Wetern blot检测多种细胞提取物，所用抗体为LDHA (C4B5) Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of MCF-7 cells using LDHA (C4B5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光检测MCF－7，所用抗体为LDHA (C4B5) Rabbit mAb兔单抗（绿），微丝用DY-554 phalloidin (红)着色，蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LDHA (C4B5) Rabbit mAb. 免疫组织化学检测石蜡包埋的人肺癌组织，所用抗体为LDHA (C4B5) Rabbit mAb兔单抗。 |
