| 货号 | 3588S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | LCP1 (D1C3) Rabbit mAb recognizes endogenous levels of total LCP1 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mammals and include L-plastin, T-plastin, and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues, while I-plastin (plastin-1) is expressed specifically in the kidney, colon, and small intestine (1-3). Research studies have shown that L-plastin (plastin-2) or lymphocyte cytosolic protein 1 (LCP1) is mainly expressed in hematopoietic cells and nonhematopoietic tumors, and increased expression correlates with metastatic progression in colon cancer cell lines (4). Investigators have found that overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T cell receptor/CD3 complex in association with the CD2 cell adhesion molecule or the CD28 receptor (6). Phosphorylation at Ser5 enhances the ability of LCP1 to bind to F-actin and increases cell motility (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from various cell lines using LCP1 (D1C3) Rabbit mAb.使用LCP1 (D1C3)Rabbit mAb对多种细胞系提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded mouse colon using LCP1 (D1C3) Rabbit mAb. 使用LCP1 (D1C3)Rabbit mAb对石蜡包埋的小鼠结肠进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon using LCP1 (D1C3) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照肽（左）或抗体特异性肽段（右）存在的条件下，使用LCP1 (D1C3)Rabbit mAb对石蜡包埋的人结肠癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LCP1 (D1C3) Rabbit mAb.使用 LCP1 (D1C3)Rabbit mAb对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using LCP1 (D1C3) Rabbit mAb.使用 LCP1 (D1C3)Rabbit mAb对石蜡包埋的人卵巢癌组织进行免疫组化分析。 |
