| 货号 | 2088S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | LEDGF (C57G11) Rabbit mAb detects endogenous levels of LEDGF protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The LEDGF (lens epithelium-derived growth factor) gene encodes splice variants P75 and P52 (1). LEDGF (C57G11) Rabbit mAb detects the 75 kDA LEDGF splice form. Both LEDGF variants have transcriptional coactivator functions that may regulate gene transcription and mRNA splicing of a variety of proteins that are essential for cell growth and survival. (2,3). LEDGF has also been identified as a factor that can, in serum free media, stimulate the growth and survival of lens epithelial cells and other cell types such as retinal photoreceptor cells, COS7 cells, skin fibroblasts, and keratinocytes (2). In addition to its function as a transcriptional coactivator, LEDGF has been shown to be involved in the pathogenesis of atopic dermatitis (4) and in the activity of HIV1 integrase. LEDGF binds chromatin throughout the cell cycle, and may act as a chromatin docking factor for HIV integrase and other lentiviral pre-integration complexes. It protects HIV integrase from degradation, and influences the pattern of viral integration into the cell genome (5). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Western blot analysis of exctracts from various cell types using LEDGF (C57G11) Rabbit mAb. 使用LEDGF (C57G11) Rabbit mAb，免疫印迹(Western blot)分析不同细胞中LEDGF (C57G11)的蛋白水平 | |
Flow cytometric analysis of Jurkat cells using LEDGF (C57G11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用LEDGF (C57G11) Rabbit mAb (蓝色)进行流式细胞仪分析Jurkat细胞。 | |
Confocal immunofluorescent analysis of HeLa cells using LEDGF (C57G11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用LEDGF (C57G11) Rabbit mAb (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LEDGF (C57G11) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 使用LEDGF (C57G11) Rabbit mAb，control peptide (左图)或antigen-specific peptide (右图)，免疫组化分析人源乳腺癌组织石蜡切片。 |
