Lefty1 (D7E3G) Rabbit mAb
货号：
12647S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 12647S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Lefty1 (D7E3G) Rabbit mAb recognizes transfected levels of total lefty1 protein. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Lefty proteins are members of the TGF-β family of cell signaling molecules that are involved in growth and development (1,2). Named for their role in left-right axis determination and their exclusive expression on the left side of the developing mouse embryo, lefty1 and lefty2 contain a cysteine-knot motif that is characteristic of TGF-β related proteins, but lack an alpha-helix and a cysteine residue critical for ligand dimerization (3). Early in vertebrate embryogenesis, lefty represses TGF-β signaling by inhibiting the phosphorylation of Smad2 following activation of the TGF-β receptor (4). Down-regulated very early upon differentiation (5), lefty proteins act as extracellular antagonists of the signaling pathway for Nodal, a TGF-β ligand critical for left-right patterning and formation of the mesoderm and endoderm (6). Similar to other members of the TGF-β superfamily, lefty proproteins undergo cleavage to release a bioactive protein (7). The biologically active 42 kDa lefty precursor and the 28 kDa polypeptide have been shown to induce MAPK activity (7). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
参考图片Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Lefty1 (hLefty1-Myc/DDK; +), using Lefty1 (D7E3G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). 空转染(-)或转染了表达Myc/DDK标记的全长人Lefty1 (hLefty1-Myc/DDK; +)质粒的293细胞提取物，使用Lefty1 (D7E3G)Rabbit mAb（上）和β-Actin (D6A8)Rabbit mAb #8457（下）进行western blot分析。 |
