| 货号 | 5930S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC |
| 目标/特异性 | LIN28A (6D1F9) Mouse mAb detects endogenous levels of total LIN28A protein. |
| 使用方法 | WB(1:1000) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 26 |
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using LIN28A (6D1F9) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用LIN28A (6D1F9) 鼠单抗（绿色）对NTERA-2细胞（左）和HeLa细胞（右）进行激光共聚焦荧光分析。使用DY-554phalloidin（红色）标记肌动蛋白丝。蓝色伪彩=DRAQ5® #4084 (荧光DNA染料). | |
Western blot analysis of extracts from various cell lines using LIN28 (6D1F9) Mouse mAb. 使用LIN28 (6D1F9) Mouse mAb兔单抗对多种细胞提取物进行Western blot 分析。 |
