| 货号 | 14906S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Lipin 1 was identified as a nuclear protein required for adipose tissue development (1). The expression of Lipin 1 is induced during adipocyte differentiation (1). The abnormal development of adipose tissues caused by mutations in the lipin 1 gene results in lipodystrophy, a condition associated with low body fat, fatty liver, hypertriglyceridemia, and insulin resistance (1). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (2-4). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in liver, and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (5). Lipin 1 is regulated by mTOR, illustrating a connection between adipocyte development and nutrient-sensing pathways (6). It also mediates hepatic insulin signaling by TORC2/CRTC2 (7). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
Confocal immunofluorescent analysis of 3T3-L1 adipocytes (differentiated, 7 days) using Lipin 1 (D2W9G) Rabbit mAb (red). Lipid droplets were labeled with BODIPY® 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Lipin 1 (D2W9G) Rabbit mAb (upper) and GAPDH (D16H11) XP®Rabbit mAb #5174 (lower). |
