| 货号 | 11965S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | LIN28B (D4H1) Rabbit mAb recognizes endogenous levels of total LIN28B protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). |
| 存放说明 | -20C |
| 计算分子量 | 32, 21 |
Western blot analysis of extracts from NTERA-2, Hep G2, and Huh7 cells using LIN28B (D4H1) Rabbit mAb. 使用LIN28B (D4H1)Rabbit mAb对NTERA-2, Hep G2, 和Huh7细胞提取物进行western blot分析。 | |
Immunoprecipitation of LIN28B from NCCIT cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or LIN28B (D4H1) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using LIN28B (D4H1) Rabbit mAb. 使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（lane2）或LIN28B (D4H1)Rabbit mAb (lane 3)对NCCIT细胞中的LIN28B蛋白进行免疫沉淀实验。Lane1是10%上样量。使用LIN28B (D4H1)Rabbit mAb进行western blot分析。 |
