| 货号 | 3050S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | LKB1 (27D10) Rabbit mAb detects endogenous levels of LKB1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5). Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
Western blot analysis of extracts from Caki-1 and COS cells using LKB1 (27D10) Rabbit mAb. 对Caki-1和COS细胞抽提液使用LKB1 (27D10) Rabbit mAb进行Western blot分析。 | |
Immunoprecipitation of LKB1 from insulin treated 293T cell extracts using LKB1 (27D10) antibody (Lane1). Lane 2: No antibody control. Lane 3: Input control. 对胰岛素处理的293T细胞裂解液使用LKB1(27D10)抗体（列1）进行LKB1免疫共沉淀分析。列2：无抗体对照。列3：输入对照。 |
