| 货号 | 2184S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/ChIP |
| 目标/特异性 | LSD1 (C69G12) Rabbit mAb detects endogenous levels of total LSD1 protein. |
| 使用方法 | WB(1:1000) IP (1:25) IHC-P (1:800) IF-IC (1:400) IHC-F (1:800) ChIP (1:50) |
| 供应商 | CST |
| 背景 | Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Confocal immunofluorescent analysis of HeLa cells using LSD1 (C69G12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用LSD1 (C69G12) Rabbit mAb (绿色)，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of cell lysates from various cell types using LSD1 (C69G12) Rabbit mAb. 使用LSD1 (C69G12) Rabbit mAb，免疫印迹(Western blot)分析不同细胞中LSD1 (C69G12)的蛋白水平 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LSD1 (C69G12) Rabbit mAb. 使用LSD1 (C69G12) Rabbit mAb，免疫组化分析人源乳腺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LSD1 (C69G12) Rabbit mAb. 使用LSD1 (C69G12) Rabbit mAb，免疫组化分析人源肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using LSD1 (C69G12) Rabbit mAb. 使用LSD1 (C69G12) Rabbit mAb，免疫组化分析人源胃癌组织石蜡切片。 | |
Immunohistochemical analysis of frozen mouse spleen using LSD1 (C69G12) Rabbit mAb. 使用LSD1 (C69G12) Rabbit mAb，免疫组化分析小鼠脾脏组织冰冻切片。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 U-937 cells and either 10 μl of LSD1 (C69G12) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP® Human CD11b Promoter Primers #14271, human SPI-1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
