| 货号 | 13519S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | LXR-β (D6M9D) Rabbit mAb recognizes endogenous levels of total LXR-β protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Liver X receptors LXR-α and LXR-β are nuclear hormone receptor superfamily members responsible for regulating expression of target genes that control cholesterol transport and metabolism (1). When bound by the oxidized derivatives of cholesterol (oxysterols), activated LXR receptors function as sterol sensors to regulate transcription of the genes involved in the cholesterol homeostasis (1,2). The LXR-α protein is expressed at high levels in rat liver, kidney, intestine, adipose, and spleen; LXR-β is more ubiquitously expressed within rat tissues (1,3). Research studies indicate that glucose binds and up-regulates the transcriptional activity of LXR-α and LXR-β (4). LXR-α and LXR-β are putative glucose sensors that integrate glucose metabolism and fatty acid biosynthesis in the liver (4). Additional studies show that female mice deficient in LXR-β develop gallbladder cancer (5). In addition, LXR-β plays a role in protecting dopaminergic neurons in a Parkinson disease model (6). |
| 存放说明 | -20C |
| 计算分子量 | 63 |
Western blot analysis of extracts from various cell lines using LXR-β (D6M9D) Rabbit mAb. 使用LXR-β (D6M9D) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human LXR-α protein (hLXR-α-Myc/DDK; +) or Myc/DDK-tagged full-length human LXR-β protein (hLXR-β-Myc/DDK; +), using LXR-β (D6M9D) Rabbit mAb (upper), DYKDDDDK (9A3) Mouse mAb #8146 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). 模拟转染(-)或转染了表达Myc/DDK标记的human LXR-α protein (hLXR-α-Myc/DDK; +)或Myc/DDK标记full-length human LXR-β protein (hLXR-β-Myc/DDK; +)质粒的293细胞提取物，使用LXR-β (D6M9D) Rabbit mAb 兔单抗(上), DYKDDDDK (9A3) Mouse mAb 鼠单抗#8146 (中), 或 GAPDH (D16H11) XP® Rabbit mAb 兔单抗#5174 (下)进行western blot分析。 |
