| 货号 | 2796S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Lyn (C13F9) Rabbit mAb detects endogenous levels of total Lyn protein. This antibody does not cross-react with any other Src-family members. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:400) |
| 供应商 | CST |
| 背景 | Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8). |
| 存放说明 | -20C |
| 计算分子量 | 56 |
Western blot analysis of cell extracts from various cell types using Lyn (C13F9) Rabbit mAb.Western免疫印迹。用Lyn (C13F9) Rabbit mAb研究各种细胞系的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lyn (C13F9) Rabbit mAb in the presence of control peptide (left) and antigen specific peptide (right).免疫组织化学染色分析石蜡包埋人扁桃体组织。在对照多肽（左图）和抗原特异性的多肽（右图）存在下所用抗体为Lyn (C13F9) Rabbit mAb 。 | |
Immunohistochemical analysis of paraffin-embedded non-hodgkins lymphoma using Lyn (C13F9) Rabbit mAb.免疫组织化学染色分析石蜡包埋非霍奇金淋巴瘤组织。所用抗体为Lyn (C13F9) Rabbit mAb。 |
