| 货号 | 12155S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2). |
| 存放说明 | -20C |
| 计算分子量 | 47, 49 (Phosphorylated) |
Western blot analysis of extracts from various cell lines, untreated (-) or treated with UV (40 mJ/cm2, 30 min recovery; +), using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).未处理（-）或使用UV (40 mJ/cm2, 30 min recovery; +)处理的多种细胞提取物，使用MAPKAPK-2 (D1E11)Rabbit mAb（上）或GAPDH (D16H11) XP®Rabbit mAb#5174（下）。 | |
Immunoprecipitation of MAPKAPK-2 from HeLa cell extracts using MAPKAPK-2 (D1E11) Rabbit mAb (lane 2), MAPKAPK-2 Antibody #3042 (lane 3), or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 4). Lane 1 is 10% input. Western blot analysis was performed using MAPKAPK-2 (D1E11) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.使用MAPKAPK-2 (D1E11)Rabbit mAb（lane2），MAPKAPK-2抗体#3042 (lane 3)，或Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 4)对HeLa细胞提取物的MAPKAPK-2进行免疫沉淀实验。Lane 1是10%上样量。MAPKAPK-2 (D1E11)Rabbit mAb用作western blot分析。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678作为二抗。 | |
Western blot analysis of cell extracts from MAPKAPK-2 wild type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). MAPKAPK-2 WT and KO MEFs were generously provided by Drs. Sandra Morandell and Michael Yaffe, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA.MAPKAPK-2野生型（WT）和敲除（KO）小鼠胚胎成纤维细胞（MEF）的提取物使用MAPKAPK-2 (D1E11)Rabbit mAb（上）或GAPDH (D16H11) XP®兔mAb #5174（下）进行western blot分析。MAPKAPK-2 WT和KO MEFs由David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA的Drs. Sandra Morandell 和Michael Yaffe提供。 |
