| 货号 | 12079S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:400) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).微染色体维持蛋白（MCM）2-7是一个由6个相关蛋白组成的家族，在DNA复制起始和延伸过程中是必须的。MCM2-7彼此结合形成异二聚体MCM复合物，被认为是DNA复制叉前进过程中的复制解旋酶(1-5).这个复合物是前复制复合物（pre-RC）的重要组成部分（1）。Cdc6和CDT1在有丝分裂/G1前期集合MCM复合物到起始识别复合物（ORC）形成pre-RC使DNA能够复制（2）。每个细胞周期中DNA只复制一次，因而帮助减少遗传变异和癌变细胞生长（3）。MCM2, MCM3, MCM4, 和MCM6亚基磷酸化可以调控MCM复合物活性起始DNA合成（6-8）。DNA复制时MCM蛋白会被移除，通过抑制pre-RC再形成而保障染色质获得许可。研究表明MCM复合物可以保护复制叉的结构并在停滞后募集检验点蛋白协助重启复制（3,9）。 |
| 存放说明 | -20C |
| 计算分子量 | 125 |
Confocal immunofluorescent analysis of HeLa cells using MCM2 (1E7) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8584. 使用MCM2 (1E7)Mouse mAb（绿色）和β-Actin (13E5)Rabbit mAb (偶联Alexa Fluor® 647) #8584对HeLa细胞进行激光共聚焦免疫荧光分析。 | |
Western blot analysis of extracts from various cell lines using MCM2 (1E7) Mouse mAb.使用MCM2 (1E7)Mouse mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of MCM2 from Jurkat cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or MCM2 (1E7) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MCM2 (1E7) Mouse mAb.使用Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2)或MCM2 (1E7)Mouse mAb(lane 3)对Jurkat细胞提取物的MCM2进行免疫沉淀实验。Lane1是10%上样量。MCM2 (1E7)Mouse mAb也被用于western blot实验。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MCM2 (1E7) Mouse mAb.使用MCM2 (1E7)Mouse mAb对石蜡包埋的人肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lymph node using MCM2 (1E7) Mouse mAb.使用MCM2 (1E7)Mouse mAb对石蜡包埋的人淋巴结进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using MCM2 (1E7) Mouse mAb.使用MCM2 (1E7)Mouse mAb对石蜡包埋的人卵巢癌组织进行免疫组化分析。 |
