| 货号 | 13978S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:800) |
| 供应商 | CST |
| 背景 | MDR1/ABCB1 belongs to the Mdr/Tap subfamily of the ATP-binding cassette transporter superfamily (1). Multidrug resistance 1 (MDR1) serves as an efflux pump for xenobiotic compounds with broad substrate specificity. MDR1 substrates include therapeutic agents such as actinomycin D, etoposide, imatinib, and doxorubicin, as well as endogenous molecules including β-amyloids, steroid hormones, lipids, phospholipids, cholesterol, and cytokines (2). Research studies have shown that MDR1 reduces drug accumulation in cancer cells, allowing the development of drug resistance (3-5). On the other hand, MDR1 expressed in the plasma membrane of cells in the blood-brain, blood-cerebral spinal fluid, or blood-placenta barriers restricts the permeability of drugs into these organs from the apical or serosal side (6,7). MDR1 is also expressed in normal tissues with excretory function such as small intestine, liver, and kidney (7). Intracellular MDR1 has been detected in the ER, vesicles, and nuclear envelope, and has been associated with cell trafficking machinery (8). Other reported functions of MDR1 include viral resistance, cytokine trafficking (9,10), and lipid homeostasis in the peripheral and central nervous system (11-13). |
| 存放说明 | -20C |
| 计算分子量 | 130-180 |
Western blot analysis of extracts from Hep G2, MCF7 and mIMCD-3 cells using MDR1/ABCB1 (E1Y7S) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower). | |
Immunohistochemical analysis of paraffin-embedded normal human liver using MDR1/ABCB1 (E1Y7S) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded normal human kidney using MDR1/ABCB1 (E1Y7S) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded Hep G2 (left) and MCF7 (right) cell pellets using MDR1/ABCB1 (E1Y7S) Rabbit mAb. |
