| 货号 | 14950S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops’ that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter, and works to sustain proper chromatin architecture during active transcription (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from Raji, HeLa, and Daudi cells using MED26 (D4B1X) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells heat shocked for 1 hr and either 5 µl of MED26 (D4B1X) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Myc Intron 1 Primers #14905, SimpleChIP® Human HSP70 Intron 1 Primers #14023, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
